| ORIGINAL ARTICLES |
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Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling |
| Ismail Ware1,2,3, Katrin Franke1,4,5, Andrej Frolov1, Kseniia Bureiko1, Elana Kysil1, Maizatulakmal Yahayu3, Hesham Ali El Enshasy3,6, Ludger A. Wessjohann1,5 |
1. Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany;
2. Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400 Sabah, Malaysia;
3. Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia;
4. Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany;
5. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 04103 Leipzig, Germany;
6. City of Scientific Research and Technology Applications, New Borg Al Arab, Alexandria 21934, Egypt |
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Abstract Piper sarmentosum Roxb. (Piperaceae) is a traditional medicinal and food plant widely distributed in the tropical and subtropical regions of Asia, offering both health and culinary benefits. In this study the secondary metabolites in different organs of P. sarmentosum were identified and their relative abundances were characterized. The metabolic profiles of leaves, roots, stems and fruits were comprehensively investigated by liquid chromatography high-resolution mass spectrometry (LC-HR-MS) and the data subsequently analyzed using multivariate statistical methods. Manual interpretation of the tandem mass spectrometric (MS/MS) fragmentation patterns revealed the presence of 154 tentatively identified metabolites, mostly represented by alkaloids and flavonoids. Principle component analysis and hierarchical clustering indicated the predominant occurrence of flavonoids, lignans and phenyl propanoids in leaves, aporphines in stems, piperamides in fruits and lignan-amides in roots. Overall, this study provides extensive data on the metabolite composition of P. sarmentosum, supplying useful information for bioactive compounds discovery and patterns of their preferential biosynthesis or storage in specific organs. This can be used to optimize production and harvesting as well as to maximize the plant’s economic value as herbal medicine or in food applications.
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| Keywords
Piper sarmentosum
LC-MS/MS
Multivariate analysis
Metabolite profiling
Secondary metabolites
Alkaloids
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| Fund:This study was supported by the German Academic Exchange Service (DAAD) as a grant scholarship and part of the Ph.D. thesis of IW. Funding program/-ID: Research Grants - Doctoral Programs in Germany, 2017/18 (57299294), ST34. |
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Corresponding Authors:
Katrin Franke,E-mail:kfranke@ipb-halle.de;Ludger A. Wessjohann,E-mail:wessjohann@ipb-halle.de
E-mail: kfranke@ipb-halle.de;wessjohann@ipb-halle.de
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Issue Date: 01 August 2024
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