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Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F |
| Yue Song1,4, Qi-Yang Li1,3, Meng-Jing Cong1,4, Xiao-Yan Pang1, Bo Chen2, Yong-Hong Liu1,4,5, Li Liao2,6, Jun-Feng Wang1,4,5 |
1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine, Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;
2. Key Laboratory for Polar Science, MNR, Polar Research Institute of China, Shanghai, 200136, China;
3. Department of Pharmacology and Therapeutics, McGill University, Montreal, H3A 0G4, Canada;
4. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China;
5. Sanya Institute of Marine Ecology and Engineering, Sanya, 572000, China;
6. School of Oceanography, Shanghai Jiao Tong University, Shanghai, 200240, China |
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Abstract Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC50 values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6-8 exhibited inhibitory activities with IC50 values of 10.88±3.05, 38.88±3.29, and 2.44±0.17 μg/mL, respectively (IC50 91.6 μM for the positive control loratadine).
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| Keywords
Nocardiopsis dassonvillei
Arctic Ocean
Phenazine
Phenoxazine
Cytotoxic
Antiallergic
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| Fund:This research was supported by the Guangdong Basic and Applied Basic Research Foundation (2021A1515011523, 2021B1515120046, 2022B1515120075), National Key Research and Development Program of China (2022YFC2807501), National Natural Science Foundation of China (grant no. 41976224), Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (2021JJLH0097, 2021CXLH0013), Hainan Provincial Natural Science Foundation of China (823CXTD393). We thank ZH Xiao, AJ Sun, XH Zheng, X Ma, and Y Zhang in the analytical facility at SCSIO for recording spectroscopic data. |
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Corresponding Authors:
Li Liao,E-mail:liaoli@pric.org.cn;Jun-Feng Wang,E-mail:wangjunfeng@scsio.ac.cn
E-mail: liaoli@pric.org.cn;liaoli@pric.org.cn;wangjunfeng@scsio.ac.cn
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Issue Date: 26 December 2023
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