Artemlavanins A and B from <i>Artemisia lavandulaefolia</i> and Their Cytotoxicity Against Hepatic Stellate Cell Line LX2

doi:10.1007/s13659-020-00254-0

Natural Products and Bioprospecting

2020, Vol. 10

Issue (4) : 243-250 DOI: 10.1007/s13659-020-00254-0

ORIGINAL ARTICLES

Artemlavanins A and B from Artemisia lavandulaefolia and Their Cytotoxicity Against Hepatic Stellate Cell Line LX2

Cheng Shen^1,2,3, Xiao-Yan Huang^1,2, Chang-An Geng^1,2, Tian-Ze Li^1,2, Shuang Tang^1,2,3, Li-Hua Su^1,2,3, Zhen Gao^1,2,3, Xue-Mei Zhang^1,2, Jing Hu^1,2, Ji-Jun Chen^1,2,3

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China

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Abstract Two new sesquiterpenoids, artemlavanins A (1) and B (3), together with fifteen known compounds (2 and 4-17) were isolated from the EtOH extract of Artemisia lavandulaefolia. The structures of new compounds were elucidated by extensive spectroscopic analyses (HRESIMS, 1D and 2D NMR) and ECD calculations. Compound 1 was a sesquiterpenoid lactone possessing a rearranged eudesmane skeleton; compounds 2-5, 6-8, 9 and 10-12 belonged to the eudesmane, guaiane, oppositane and farnesane sesquiterpenoids, respectively; compounds 13-17 were the phenyl derivatives with a 4-hydroxy-acetophenone moiety. Twelve compounds (1-3, 5-7, 10-12, 14, 15 and 17) displayed cytotoxicity against hepatic stellate cell line LX2 (HSC-LX2) with IC₅₀ values ranging from 35.1 to 370.3 μM. Compounds 2, 7, 10-12 and 17 exhibited the stronger cytotoxicity than silybin (IC₅₀, 169.6 μM) with IC₅₀ values of 82.1, 35.1, 95.0, 83.8, 81.6 and 90.1 μM. Compound 7 as the most active one showed significant inhibition on the deposition of human collagen type I (Col I), human hyaluronic acid (HA) and human laminin (HL) with IC₅₀ values of 10.7, 24.5 and 13.3 μM.

Keywords Artemisia lavandulaefolia Sesquiterpenoids Artemlavanins Cytotoxicity HSC-LX2

Fund:This work was supported by the Yunnan Wanren Project (YNWR-KJLJ-2019-002), the Program of Yunling Scholarship, the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province, and the Youth Innovation Promotion Association, CAS (2013252).

Corresponding Authors: Ji-Jun Chen E-mail: chenjj@mail.kib.ac.cn

Issue Date: 19 August 2020

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	Cheng Shen
	Xiao-Yan Huang
	Chang-An Geng
	Tian-Ze Li
	Shuang Tang
	Li-Hua Su
	Zhen Gao
	Xue-Mei Zhang
	Jing Hu
	Ji-Jun Chen

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Cheng Shen,Xiao-Yan Huang,Chang-An Geng, et al. Artemlavanins A and B from Artemisia lavandulaefolia and Their Cytotoxicity Against Hepatic Stellate Cell Line LX2[J]. Natural Products and Bioprospecting, 2020, 10(4): 243-250.

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http://npb.kib.ac.cn/EN/10.1007/s13659-020-00254-0 OR http://npb.kib.ac.cn/EN/Y2020/V10/I4/243

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