| ORIGINAL ARTICLES |
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Novel neo-clerodane diterpenoids from Teucrium quadrifarium and their anti-ferroptosis effect |
| Huan Wang1,2, Han-Fei Liu1,2, Xiao-Qiao Yang1,2, Yu-Qiong Liao1,2, Fen-Cong Pan1,2, Jin-Yu Li1,2, Hua-Yong Lou1,2, Wei-Dong Pan1 |
1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, People’s Republic of China;
2. Natural Products Research Center of Guizhou Province, Guiyang, 550014, People’s Republic of China |
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Abstract Teucrifarides A–D (1–4), four previously unreported neo-clerodane-type diterpenoids, combined with sixteen known analogs (5–20), were purified from Teucrium quadrifarium. The absolute forma of compounds 1–4 were determined via spectroscopic and ECD calculation methods, together with X-ray crystallography experiments. Among them, compound 1 possessed a 5,20-epoxy ring featuring a unique cage-like 12-oxatricyclo [5.3.2.01,6]undecane skeleton. Meanwhile, 2 incorporated a 6,20-epoxy ring with a novel 12-oxatricyclo [6.2.2.02,7]undecane skeleton. Compounds 1 and 12 exhibited significant inhibitory effects against HT-22 cells ferroptosis induced by RSL3, with EC50 values of 11.8±1.0 μM, and 4.52±1.24 μM, respectively. Moreover, ROS accumulation in HT22 cells treated with compound 1 was also observed.
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| Keywords
Teucrium quadrifarium
Lamiaceae
Neo-clerodane
Ferroptosis inhibitory activity
ROS
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| Fund:This work was financially supported by the National Natural Science Foundation of China (32100322, 32460112, 32060100); and the Science and Technology Department of Guizhou Province (QKHZC[2022]019). |
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Corresponding Authors:
Jin-Yu LI,E-mail:lijinyu20080@126.com;Hua-Yong LOU,E-mail:loouhy@126.com;Wei-Dong PAN,E-mail:wdpan@163.com
E-mail: lijinyu20080@126.com;loouhy@126.com;wdpan@163.com
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Issue Date: 15 February 2025
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