| ORIGINAL ARTICLE |
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Grewiifopenes A-K, bioactive clerodane diterpenoids from Casearia grewiifolia Vent. |
| Phanruethai Pailee1, Paratchata Batsomboon1, Wiriya Yaosanit1, Theerawat Thananthaisong2, Chulabhorn Mahidol1,3, Poonsakdi Ploypradith1,3,4, Nanthawan Reuk-ngam1, Panita Khlaychan1, Supanna Techasakul1, Somsak Ruchirawat1,3,4, Vilailak Prachyawarakorn1 |
1. Laboratory of Natural Products, Medicinal Chemistry and Organic Synthesis, Chulabhorn Research Institute, Bangkok, 10210, Thailand;
2. Department of National Parks, Wildlife and Plant Conservation, Forest Herbarium, Bangkok, 10900, Thailand;
3. Program in Chemical Sciences, Chulabhorn Graduate Institute, Bangkok, 10210, Thailand;
4. Center of Excellence on Environmental Health and Toxicology (EHT), Office of the Permanent Secretary (OPS), Ministry of Higher Education, Science, Research and Innovation (MHESI), Bangkok, 10400, Thailand |
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Abstract Eleven novel clerodane-type diterpenoids, grewiifopenes A-K (1-4 and 12-18), along with nine known compounds (5-11, 19, and 20) were purified from the dichloromethane extract of the twigs and stems of Casearia grewiifolia Vent. (Salicaceae). Their spectroscopic data, including the NMR, HRESIMS, and electronic circular dichroism calculations were employed to completely characterize and elucidate the chemical structures and absolute configurations. The clerodane diterpenoids possessing a 6-OH group and no substitution at C-7 exhibited greater cytotoxic activity than others, with their IC50 values ranging from 0.3 to 2.9 μM. Isocaseamembrin E (7) exhibited antibacterial activity against Staphylococcus aureus, while isocaseamembrin E (7), corymbulosin X (8), caseargrewiin A (9), kurzipene A (10), and balanspene F (11) exhibited antibacterial activity against Bacillus cereus.
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| Keywords
Casearia grewiifolia Vent.
Salicaceae
Clerodane diterpenoids
Cytotoxic activity
Antibacterial activity
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| Fund:The grants from Thailand Science Research and Innovation (TSRI) and Chulabhorn Research Institute (grant numbers 48296/4691995) and Center of Excellence on Environmental Health and Toxicology (EHT), OPS, Ministry of Higher Education, Science, Research and Innovation were supported the research funding. |
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Corresponding Authors:
Vilailak Prachyawarakorn,E-mail:vilailak@cri.or.th
E-mail: vilailak@cri.or.th
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Issue Date: 13 December 2024
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