| ORIGINAL ARTICLES |
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Discovery of structurally diverse sesquiterpenoids from Streptomyces fulvorobeus isolated from Elephas maximus feces and their antifungal activities |
| Lu Cao1, Jun-Feng Tan1, Zeng-Guang Zhang1, Jun-Wei Yang1, Yu Mu1, Zhi-Long Zhao2, Yi Jiang3, Xue-Shi Huang1, Li Han1 |
1. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, China;
2. Pharmacological Laboratory, Liaoning Provincial Institute of Drug Inspection and Testing, Shenyang 110036, China;
3. Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China |
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Abstract Thirty-six structurally diverse sesquiterpenoids, including caryolanes (1-12), germacranes (13-16), isodaucane (17), cadinanes (18-22), epicubenols (23, 24), oplopanane (25), pallenanes (26, 27), and eudesmanes (28-36), were isolated from the fermentation broth of Streptomyces fulvorobeus derived from Elephas maximus feces. Pallenane is a kind of rarely reported sesquiterpene with a distinctive C5/C3 bicyclic skeleton and was firstly found from microbial source. The structures of fifteen new compounds (1-4, 13-15, 17, 18, 22, 23, 25-28) were established through detailed spectroscopic data analysis, which included data from experimental and calculated ECD spectra as well as Mosher’s reagent derivative method. Compound 34 exhibited moderate antifungal activity against Cryptococcus neoformans and Cryptococcus gattii with MIC values of 50 μg/mL. It effectively inhibited biofilm formation and destroyed the preformed biofilm, as well as hindered the adhesion of Cryptococcus species. The current work would enrich the chemical diversity of sesquiterpenoid family.
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| Keywords
Sesquiterpenoids
Streptomyces fulvorobeus
Fermentation
Antifungal activity
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| Fund:This work was supported by National Natural Science Foundation of China (Grant No. 32060001), the Fundamental Research Funds for the Central Universities, China (No. N2320001) and the Construction Project of Liaoning Provincial Key Laboratory, China (2022JH13/10200026). |
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Corresponding Authors:
Yi Jiang,E-mail:jiangyi@ynu.edu.cn;Xue-Shi Huang,E-mail:huangxs@mail.neu.edu.cn;Li Han,E-mail:hanli@mail.neu.edu.cn
E-mail: jiangyi@ynu.edu.cn;huangxs@mail.neu.edu.cn;hanli@mail.neu.edu.cn
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Issue Date: 13 December 2024
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