| ORIGINAL ARTICLES |
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Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response |
| Tian Lan1, Wen Wang2, De-Lian Huang3, Xi-Xi Zeng1, Xiao-Xiao Wang4, Jian Wang4, Yu-Hua Tong1,5, Zhu-Jun Mao5,6, Si-Wei Wang1,5 |
1. The Joint Innovation Center for Health and Medicine, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, No. 100 Minjiang Road, Quzhou, 324000, China;
2. Preventive Treatment Center, Zhejiang Chinese Medical University Affiliated Four-provinces Marginal Hospital of Traditional Chinese Medicine, Quzhou Hospital of Traditional Chinese Medicine, Quzhou, 324000, China;
3. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, 310053, China;
4. Department of Drug Analysis Center, Quzhou Institute for Food and Drug Control, Quzhou, 324000, China;
5. Department of Ophthalmology, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou, 324000, China;
6. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou, 310053, China |
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Abstract Quzhou Aurantii Fructus (QAF) has a long history as a folk medicine and food for the treatment of liver diseases. While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF, the potential preventative effects afforded by essential oil components present within QAF remains enigmatic. In this study, we prepared Quzhou Aurantii Fructus essential oil (QAFEO) and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine (LPS/D-GalN) induced acute liver failure (ALF) mouse models. Using RNA-sequence (RNA-seq) analysis, we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways. QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1 (TBK1), TGF-beta activated kinase 1 (TAK1), interferon regulatory factor 3 (IRF3), and the activation of mitogen activated kinase-like protein (MAPK) and nuclear factor-kappa B (NF-κB) pathways in vivo and in vitro. Importantly, QAFEO substantially reduced myeloid differentiation primary response gene 88 (MyD88)- toll-like receptor 4 (TLR4) interaction levels. Moreover, 8 compounds from QAFEO could directly bind to REAL, TAK1, MyD88, TBK1, and IRF3. Taken together, the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response.
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| Keywords
Quzhou Aurantii Fructus
Essential oil
Acute liver failure
LPS
Inflammation
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| Fund:This work was supported by Zhejiang Provincial Natural Science Foundation of China [LQ22H270014], the Chinese medicine science foundation of Zhejiang Province, China [2020ZA120], Quzhou technology projects, China [2022K53, 2023K113, 2023K120], Science and technology project of Zhejiang Provincial Drug Administration [2021013]. |
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Corresponding Authors:
Yu-Hua Tong,E-mail:yuhuatong@126.com;Zhu-Jun Mao,E-mail:maozhujun0107@163.com;Si-Wei Wang,E-mail:358031289@qq.com
E-mail: yuhuatong@126.com;maozhujun0107@163.com;358031289@qq.com
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Issue Date: 03 November 2023
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