| ORIGINAL ARTICLES |
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Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier |
| Qing Tao1, Jinhua Zhang2, Qiao liang1, Shiyu Song1, Shuxia Wang2, Xiaoming Yao2, Qian Gao1, Lei Wang1,3 |
1. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, 210093, Jiangsu, China;
2. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China;
3. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China |
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Abstract More and more evidence suggests that puerarin, a potential remedy for gut inflammation, may have an ameliorative effect on sleep disturbances. However, the relationship between puerarin and sleep disruption has not been extensively researched. This study aims to explore the role and mechanisms of puerarin in improving sleep disorders. We established a light-induced sleep disorder model in mice and assessed the effects of puerarin on cognitive behavior using open field and water maze tests. Pathological detection demonstrated that sleep disturbances resulted in observable damage to the liver, lung, and kidney. Puerarin reversed multi-organ damage and inflammation. Further, puerarin activated paneth cells, resulting in increased lysozyme and TGF-β production, and stimulating intestinal stem cell proliferation. Puerarin also effectively inhibited the expression of F4/80, iNOS, TNF-α, and IL-1β in the small intestine, while it increased Chil3, CD206, and Arg-1 levels. Moreover, puerarin treatment significantly decreased P-P65, TLR4, Bcl-xl, and cleaved caspase-3 protein levels while increasing barrier protein levels, including ZO-1, Occludin, Claudin 1 and E-cadherin suggesting a reduction in inflammation and apoptosis in the gut. Overall, puerarin diminished systemic inflammation, particularly intestinal inflammation, and enhanced intestinal barrier integrity in mice with sleep disorders. Our findings suggest a potential new therapeutic pathway for sleep disorders.
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| Keywords
Intestinal inflammation
Intestinal mucosal barrier
Light-induced sleep disorder
Puerarin
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| Fund:We thank all involved for their participation and technical support. We also thank the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University and the Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese medicine, Jiangsu Province Academy of Traditional Chinese Medicine. The authors wish to express their gratitude for the financial support received from the National Key Research and Development Program of China (No: 2020YFC2005300, No: 2021YFC2009101), Open/Independent Project of the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, and Jiangsu Province Postgraduate Scientific Research Innovation Project (KYCX22-0183). |
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Corresponding Authors:
Qian Gao,E-mail:qian_gao@nju.edu.cn;Lei Wang,E-mail:wlei@nju.edu.cn
E-mail: qian_gao@nju.edu.cn;wlei@nju.edu.cn
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Issue Date: 08 October 2023
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