| ORIGINAL ARTICLES |
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Anti-inflammatory and Cytotoxic Triterpenes from the Rot Roots of Panax notoginseng |
| Jia-Huan Shang1,2, Guo-Wei Xu1,2, Hong-Tao Zhu1,3, Dong Wang1,3, Chong-Ren Yang1, Ying-Jun Zhang1,3 |
1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China;
3 Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China |
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Abstract Four new protopanaxatriol-type triterpenes (1-2) and glucosides (3-4), were isolated from the rot roots of Panax notoginseng (Burk.) Chen, along with four known ones (5-8). Their structures were elucidated on the basis of extensive spectroscopic analysis (HRESIMS, NMR, UV, IR, and OR) and acidic hydrolysis. The possible transformation pathway of these compounds were also speculated from ginsenoside Rg1. Compound 1, with a unique α,β-unsaturated ketene in its side chain, showed significant inhibitory effects against NO production on Murine macrophage cells (IC50=4.12 ±0.20 μM) and comparable cytotoxicities against five human cancer cell lines (myeloid leukemia HL-60, lung cancer A-549 cells, hepatocellular carcinoma SMMC7721, breast cancer MCF-7, and colon cancer SW480) to positive control, cisplatin (DDP).
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| Keywords
Panax notoginseng
Rot root
Triterpenes and saponins
Inhibition on NO production
Cytotoxicity
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| Fund:The authors are grateful to the members of the analytical and bioactivity screening groups at the State Key Laboratory of Phytochemistry and Plant Resources in West China, KIB, CAS, for measuring the spectroscopic data and cytotoxic assay. This work was supported by the Major Science and Technique Programs in Yunnan Province (2016ZF001-001), the Science and Technology Planning Project of Yunnan Province (2013FC008) and Yung-Chi Cheng academician workstation of Yunnan provincial academy of science and technology (2015IC017). |
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Corresponding Authors:
Ying-Jun Zhang
E-mail: zhangyj@mail.kib.ac.cn
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Issue Date: 09 August 2019
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