In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

doi:10.1007/s13659-018-0155-0

Natural Products and Bioprospecting

2018, Vol. 8

Issue (2) : 97-105 DOI: 10.1007/s13659-018-0155-0

ORIGINAL ARTICLES

In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

Manar Adam¹, Gihan O. M. Elhassan¹, Sakina Yagi¹, Fatma Sezer Senol², Ilkay Erdogan Orhan², Abdel Azim Ahmed¹, Thomas Efferth³

1 Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan;
2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;
3 Department of Pharmaceutical Biology, Johannes Gutenberg University, 55128 Mainz, Germany

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Abstract We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically. Cytotoxicity towards CCRF-CEM cells was evaluated by the resazurin reduction assay. Geranium favosum followed by Kalanchoe glaucescens, Malva parviflora, Aizoon canariense, and Coleus barbatus, respectively, possessed the highest antioxidant activity among the studied plants. Chrozophora oblongifolia and K. glaucescens exerted considerable cytotoxicity against CCRF-CEM leukemia cells. These plants may serve as source for the further development of natural antioxidant and antitumor agents.

Keywords Antioxidant activity Cytotoxicity Pharmacognosy Plant extract

Fund:The authors would like to acknowledge Prof. Maha Kordofani (Botany Department, Faculty of Science, University of Khartoum) for the identification of the plants. We are grateful to a stipend of the German Academic Exchange Service (DAAD) to Gihan Elhaboob.

Corresponding Authors: Thomas Efferth E-mail: efferth@uni-mainz.de

Issue Date: 26 April 2018

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	Manar Adam
	Gihan O. M. Elhassan
	Sakina Yagi
	Fatma Sezer Senol
	Ilkay Erdogan Orhan
	Abdel Azim Ahmed
	Thomas Efferth

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Manar Adam,Gihan O. M. Elhassan,Sakina Yagi, et al. In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan[J]. Natural Products and Bioprospecting, 2018, 8(2): 97-105.

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http://npb.kib.ac.cn/EN/10.1007/s13659-018-0155-0 OR http://npb.kib.ac.cn/EN/Y2018/V8/I2/97

1. H. Khalid, W.E. Abdalla, H. Abdelgadir, T. Opatz, T. Efferth, Nat. Prod. Bioprospect. 2, 92-103 (2012)
2. U.P. Albuquerque, P.M. de Medeiros, M.A. Ramos, W.S.F. Júnior, A.L.P. Nascimento, W.M.T. Avilez, G.J. de Melo, Revist. Brasil. Farmacogn. 24, 110-115 (2014)
3. I.K. Khafagi, A. Dewedar, J. Ethnopharmacol. 71, 365-376 (2000)
4. C. Gyllenhaal, M.R. Kadushin, B. Southavong, K. Sydara, S. Bouamanivong, M. Xaiveu, L.T. Xuan, N.T. Hiep, N.V. Hung, P.K. Loc, L.X. Dac, T.Q. Bich, N.M. Cuong, H.M. Ly, H.J. Zhang, S.G. Franzblau, H. Xie, M.C. Riley, B.G. Elkington, H.T. Nguyen, D.P. Waller, C.Y. Ma, P. Tamez, G.T. Tan, J.M. Pezzuto, D.D. Soejarto, Pharm. Biol. 50, 30-41 (2012)
5. P. Rajendran, N. Nandakumar, T. Rengarajan, R. Palaniswami, E.N. Gnanadhas, U. Lakshminarasaiah, J. Gopas, I. Nishigaki, Clin. Chim. Acta 436, 332-347 (2014)
6. T.L. Sampaio, R.R. Menezes, M.F. da Costa, G.C. Meneses, M.C. Arrieta, A.J.C. Filho, G.B. de Morais, A.B. Libório, R.S. Alves, J.S. Evangelista, A.M. Martins, Phytomedicine 23, 1843-1852 (2016)
7. F.S. Youssef, M.L. Ashour, M. Sobeh, H.A. El-Beshbishy, A.N. Singab, M. Wink, Phytomedicine 23, 1484-1493 (2016)
8. K. Suchal, S. Malik, N. Gamad, R.K. Malhotra, S.N. Goyal, J. Bhatia, D.S. Arya, Phytomedicine 23, 1401-1408 (2016)
9. L. Subedi, B.P. Gaire, M.H. Do, T.H. Lee, S.Y. Kim, Phytomedicine 23, 872-881 (2016)
10. R. Simeonova, V. Vitcheva, I. Krasteva, P. Zdraveva, S. Konstantinov, I. Ionkova, Phytomedicine 23, 483-490 (2016)
11. J. Ferlay, H.R. Shin, F. Bray, D. Forman, C. Mathers, D.M. Parkin, Int. J. Cancer 127, 2893-2917 (2010)
12. IARC, Cancer Incidence and Mortality Worldwide, vol. 10 (IARC Cancer Base, 2011)
13. I.L. Hsin, S.C. Wang, J.R. Li, T.C. Ciou, C.H. Wu, H.M. Wu, J.L. Ko, Phytomedicine 23, 1566-1573 (2016)
14. S. Umsumarng, P. Pitchakarn, S. Yodkeeree, W. Punfa, S. Mapoung, R.A. Ramli, S.G. Pyne, P. Limtrakul, Phytomedicine 34, 182-190 (2017)
15. E. Juengel, S. Euler, S. Maxeiner, J. Rutz, S. Justin, F. Roos, W. Khoder, K. Nelson, W.O. Bechstein, R.A. Blaheta, Phytomedicine 27, 1-7 (2017)
16. F. White, in The Vegetation of Africa:a Descriptive Memoir to Accompany the Unesco/AETFAT/UNSO Vegetation Map of Africa, Natural Resource Res, vol. 20 (UNESCO, Paris, 1983)
17. B.M. Hybertson, B. Gao, S.K. Bose, J.M. McCord, Mol. Aspects Med. 32, 234 (2011)
18. R.L. Prior, X.L. Wu, K. Schaich, J. Agricult, Food Chem. 53, 4290-4302 (2005)
19. M. Ismail, M. Ibrar, Z. Iqbal, J. Hussain, H. Hussain, M. Ahmed, A. Ejaz, M.I. Choudhary, Rec. Nat. Prod. 3, 193-197 (2009)
20. O.A.J. Afolayan, M. Aboyade, M.O. Sofidiya, J. Biol. Sci. 8, 945-949 (2008)
21. A.A. Al-Laith, J. Alkhuzai, A. Freije, Arab. J. Chem. (2015). https://doi.org/10.1016/j.arabjc.2015.03.004
22. J.S. Mossa, M.A. Al-Yahya, I.A. Al-Meshal, Fitoterapia 64, 147-158 (1993)
23. F.S. Al-Saleh, H.H. Ali, M. Mirza, Fitoterapia 64, 251-256 (1993)
24. V. Shah, S.V. Bhat, B.S. Bajwa, H. Dornauer, N.J. de Souza, Planta Med. 39, 183-185 (1980)
25. B. Gabetta, G. Zini, B. Danieli, Phytochemistry 28, 859-862 (1980)
26. A. Karagöz, N. Doruöz, Z. Zeybek, A. Aslan, J. Med. Plants Res. 3, 1034-1039 (2009)
27. O.T. Cetin, M. Akarsu, E. Burunkaya, O. Kesmez, E. Arpac, H. Cetin, Egypt J. Biol. Pest Contr. 23, 287-290 (2013)
28. R. Milad, S. El-Ahmady, A. Singab, Eur. J. Med. Plants 4, 86-104 (2014)
29. J. Boik, Natural Compounds in Cancer Therapy (Oregon Medical Press, Princeton, 2001)
30. L.K. Omosa, J.O. Midiwo, V.A. Masila, B.M. Gisacho, R. Munayi, F. Kamakama, K.B. Chemutai, G. Elhaboob, M.E.M. Saeed, S. Hamdoun, V. Kuete, T. Efferth, J. Ethnopharmacol. 179, 177-196 (2016)
31. V. Kuete, A.T. Mbaveng, E.C. Nono, C.C. Simo, M. Zeino, A.E. Nkengfack, T. Efferth, Phytomedicine 23, 856-863 (2016)
32. V. Kuete, A.T. Mbaveng, L.P. Sandjo, M. Zeino, T. Efferth, Phytomedicine 33, 62-68 (2017)
33. S. Roenqsumran, K. Musikul, A. Petsom, T. Vilaivan, P. Sanqvanich, S. Pornpakakul, S. Puthong, C. Chaichantipyuth, N. Jaiboon, N. Chaichit, Planta Med. 68, 274-277 (2002)
34. P. Wu, Y. Hsu, T. Wu, K.F. Bastow, K. Lee, Org. Lett. 8, 5207-5210 (2006)
35. U. Supratman, T. Fujita, K. Akiyama, H. Hayashi, A. Murakami, H. Sakai, K. Koshimizu, H. Ohigashi, Biosci. Biotechnol. Biochem. 5, 947-949 (2001)
36. L.S. Aisyah, Y.F. Yun, A.A. Abdilla, T. Herlina, E. Julaeha, U. Supratman, Akta Kimindo 1, 1-4 (2016)
37. D. Slamenova, E. Horvathova, Neoplasma 60, 343-354 (2013)
38. R. Dianita, I. Jantan, Pharm. Biol. 55, 1715-1739 (2017)
39. R. Schuster, L. Zeindl, W. Holzer, N. Khumpirapang, S. Okonogi, H. Viernstein, M. Mueller, Phytomedicine 24, 157-165 (2017)
40. K. Vijayaraghavan, J. Rajkumar, S.N. Bukhari, B. Al-Sayed, M.A. Seyed, Mol. Med. Rep. 15, 1007-1016 (2017)
41. I.B. Abubakar, K.H. Lim, T.S. Kam, H.S. Loh, Phytomedicine 30, 74-84 (2017)
42. W.W. Chao, C.C. Su, H.Y. Peng, S.T. Chou, Phytomedicine 34, 191-201 (2017)
43. M.S. Blois, Nature 181, 1199-1200 (1958)
44. K. Schlesier, M. Harvat, V. Bohm, R. Bitsch, Free Radic. Res. 36, 177-187 (2002)
45. M.T. Chua, Y.T. Tung, S.T. Chang, Bioresour. Technol. 99, 1918-1925 (2008)
46. M. Oyaizu, Jpn. J. Nutr. Diet. 44, 307-315 (1986)
47. G. Falcioni, D. Fedeli, L. Tiano, I. Calzuola, L. Mancinelli, V. Marsili, G. Gianfranceschi, J. Food Sci. 67, 2918-2922 (2002)
48. V. Kuete, A.T. Mbaveng, E.C. Nono, C.C. Simo, M. Zeino, A.E. Nkengfack, T. Efferth, Phytomedicine 23, 856-863 (2016)
49. V. Kuete, A.T. Mbaveng, L.P. Sandjo, M. Zeino, T. Efferth, Phytomedicine 33, 62-68 (2017)
50. V.L. Singleton, J.A. Rossi Jr., Am. J. Enol. Vitic. 16, 144-158 (1965)
51. R. Woisky, A. Salatino, J. Agric. Res. 37, 99-105 (1998)
52. M. Braun, H. Burgstaller, A.M. Hamdoun, H. Walter, Common weeds of central Sudan (Verlag Josef Margraf. Germany, 1991)
53. M.E.M. Saeed, H. Abdelgadir, Y. Sugimoto, H.E. Khalid, T. Efferth, J. Ethnopharmacol. 174, 644-658 (2015)
54. G.E.B. El Ghazali, M.S. El-Tohami, A.A.B. El-Egami, Medicinal plants of the Sudan. Part Ⅲ. Medicinal plants of the White Nile provinces (Khartoum University Press, Khartoum, Sudan, 1994)
55. M.A. Ahmed, A.A. Mariod, I.H. Hussein, A. Kamal-eldin, Int. J. Pharm. Rev. Res. 4, 227-232 (2014)
56. G.E.B. El Ghazali, M.S. El Tohami, A.A. El Egami, W.E. Abdalla, M. Galal, Medicinal Plants of the Sudan Part IV. Medicinal Plants of North Kordofan (Omdurman Islamic University Press, Khartoum, Sudan, 1997)

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