| Original article |
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Differential Effect of Artemisinin Against Cancer Cell Lines |
| Mounir Tilaoui, Hassan Ait Mouse, Abdeslam Jaafari, Abdelmajid Zyad |
| Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, P. Box 523, 23000 Béni-Mellal, Morocco |
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Abstract The present study aims at defining the differential cytotoxicity effect of artemisinin toward P815(murin mastocytoma) and BSR(kidney adenocarcinoma of hamster) cell lines. Cytotoxicity was measured by the growth inhibition using MTT assay. These in vitro cytotoxicity studies were complemented by the determination of apoptotic DNA fragmentation and Annexin V-streptavidin-FITC assay. Furthermore, we examined the in vitro synergism between artemisinin and the chemotherapeutic drug, vincristin. The in vivo study was investigated using the DBA2/P815(H2d) mouse model. While artemisinin acted on both tumor cell lines, P815 was much more sensitive to this drug than BSR cells, as revealed by the respective IC50 values(12 μM for P815 and 52 μM for BSR cells). On another hand, and interestingly, apoptosis was induced in P815 but not induced in BSR. These data, reveal an interesting differential cytotoxic effect, suggesting the existence of different molecular interactions between artemisinin and the studied cell lines. In vivo, our results clearly showed that the oral administration of artemisinin inhibited solid tumor development. Our study demonstrates that artemisinin caused differential cytotoxic effects depending not only on the concentration and time of exposure but also on the target cells.
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| Keywords
Artemisinin
Cytotoxicity
Apoptosis/necrosis
Synergism
Antitumor activity
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| Fund:The authors would like to thank Prof. Zacharie Brahmi(The Children's Hospital of Indianapolis, USA), for having reviewed the manuscript. The work was funded by a grant from the CNRST(PROTARSIII, D61/07), Rabat, Morocco. |
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Issue Date: 11 February 2018
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