| ORIGINAL ARTICLES |
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Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine |
| Yun Zhao1,3, Qiong Jin1,3, Zi-Jiao Wang1,3, Xing-Yu Tao1,3, Xiao-Dong Luo1,2 |
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China;
2. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
3. University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China |
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Abstract Worldwide, fruit is an indispensable treasure house of nutrition for human beings, occupying a vital position of human diet. Postharvest fruit storage requires efficient antifungal agents to control Botrytis cinerea, which is a vital postharvest disease affecting fruit and leading to enormous losses. However, with the enormous abuse of existing antifungal drugs, the problem of drug-resistant fungi is imminent, making the controlling diseases caused by pathogenic fungi even more challenging. Drug repurposing is an efficient alternative method, we evaluated a well-known antifungal chemical, terbinafine, against the agricultural pathogen, B. cinerea in vitro, as a result, terbinafine showed strong antifungal activity. Furthermore, the in vivo antifungal activity of terbinafine was evaluated, the results showed that terbinafine could reduce the decay area on grapes. Terbinafine could disrupt the cell membrane integrity, increase cell membrane permeability, and eventual cell death of B. cinerea. In addition, terbinafine reduced decay incidence, and weight loss and maintained the soluble solids, titratable acidity, ascorbic acid, total phenolic, and malondialdehyde content during the storage period of grapes. Overall, terbinafine could be an antifungal preservative for postharvest table grapes fresh-keeping.
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| Keywords
Terbinafine
Botrytis cinerea
Antifungal activity
Cell membrane
Grape preservation
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| Fund:The authors gratefully acknowledge the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006) financial support. We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support. We are grateful to the State Key Laboratory for Conservation and Utilization of Bio-Resources in Kunming, Yunnan Province, P. R. China for providing fungal cultures. |
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Corresponding Authors:
Xiao-Dong Luo,E-mail:xdluo@mail.kib.ac.cn
E-mail: xdluo@mail.kib.ac.cn
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Issue Date: 08 October 2023
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