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Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit |
| Xiao-Na Wang1, Zhao-Jie Wang1, Yun Zhao2, Huan Wang1, Mei-Ling Xiang1, Yang-Yang Liu1, Li-Xing Zhao1, Xiao-Dong Luo1,2 |
1. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, Kunming, 650201, People's Republic of China |
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Abstract Postharvest pathogens can affect a wide range of fresh fruit and vegetables, including grapes, resulting in significant profit loss. Isoquinoline alkaloids of Mahonia fortunei, a Chinese herbal medicine, have been used to treat infectious microbes, which might be effective against postharvest pathogens. The phytochemical and bioactive investigation of this plant led to the isolation of 18 alkaloids, of which 9 compounds inhibited the growth of Botrytis cinerea and 4 compounds against Penicillium italicum. The antifungal alkaloids could change the mycelium morphology, the total lipid content, and leak the cell contents of B. cinerea. Furthermore, the two most potent antifungal alkaloids, berberine (13) completely inhibited effect on gray mold of table grape at 512 mg L-1, while jatrorrhizine (18) exhibited an inhibition rate > 90% on grape rot at the same concentration, with lower cytotoxicity and residue than chlorothalonil, which suggested that ingredients of M. fortunei might be a low-toxicity, low-residue, eco-friendly botanical fungicide against postharvest pathogens.
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| Keywords
Mahonia fortunei
Chemical constituents
Botrytis cinerea
Penicillium italicum
Anti-postharvest pathogens
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| Fund:This work was supported by NSFC (32170405), the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006). We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support. |
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Corresponding Authors:
Li-Xing Zhao,E-mail:lixingzhao@ynu.edu.cn;Xiao-Dong Luo,E-mail:xdluo@ynu.edu.cn
E-mail: lixingzhao@ynu.edu.cn;xdluo@ynu.edu.cn
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Issue Date: 18 May 2023
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