Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in <i>Boreostereum vibrans</i> of Basidiomycota

doi:10.1007/s13659-016-0096-4

Natural Products and Bioprospecting

2016, Vol. 6

Issue (3) : 167-171 DOI: 10.1007/s13659-016-0096-4

Original article

Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

Hui Zhou^1,2, Yan-Long Yang^1,2, Jun Zeng^1,2, Ling Zhang¹, Zhi-Hui Ding¹, Ying Zeng¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

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Abstract Sesquiterpenoids are very common among natural products. A large number of sesquiterpene synthase genes have been cloned and functionally characterized. However, until now there is no report about the δ-cadinol synthase predominantly forming d-cadinol (syn. torreyol) from farnesyl diphosphate. Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans. This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B. vibrans. Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B. vibrans. The crude and purified recombinant enzymes, when incubating with farnesyl diphosphate as substrate, gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase.

Keywords Delta-cadinol Sesquiterpene synthase Biosynthesis GC-MS Fungi

Fund:This work was supported by a grant from the National Natural Science Foundation of China (21572237);We are grateful to Professor Jian-Xin Pu (Kunming Institute of Botany) for his helpful suggestion

Issue Date: 08 February 2018

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Hui Zhou,Yan-Long Yang,Jun Zeng, et al. Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota[J]. Natural Products and Bioprospecting, 2016, 6(3): 167-171.

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http://npb.kib.ac.cn/EN/10.1007/s13659-016-0096-4 OR http://npb.kib.ac.cn/EN/Y2016/V6/I3/167

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