A reporter gene system for screening inhibitors of Wnt signaling pathway

doi:10.1007/s13659-012-0094-0

Natural Products and Bioprospecting

2013, Vol. 3

Issue (1) : 24-28 DOI: 10.1007/s13659-012-0094-0

Regular Article

A reporter gene system for screening inhibitors of Wnt signaling pathway

Xing-Yao LI^a,b, Yuan-Yuan WANG^a,c, Chun-Mao YUAN^a, Xiao-Jiang HAO^a, Yan LI^a

a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
b University of Chinese Academy of Sciences, Beijing 100049, China;
c School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Shandong 264209, China

Abstract
References
Related Articles
Metrics

Download: PDF(1989 KB) HTML ()
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract Abnormal activation of canonical Wnt signaling has been associated with various types of cancer. Inhibitory reagents targeting the Wnt signaling have great potential to inhibit the growth of relevant tumors. Here we generated a cell-based screening strategy for identification of antagonists of the Wnt/β-catenin signaling pathway. Stable expression wnt3a was generated in HEK293 cell line, which harbors dual-luciferase reporters. The Wnt signaling in the stably transfected cell line was proved to be very sensitive to (-)-epigallocatechin-3-gallate (EGCG) and lithium chloride (LiCl) treatment, respectively. Natural compounds were screened and a couple of novel inhibitory modulators of the Wnt signaling pathway were obtained.

Keywords Wnt signaling natural compounds dual-luciferase reporter tumor

Fund:We thank Dr. B. Mao for TopFlash and renilla plasmids, and Dr. H. Zhou for scientific and technological comments and advice on the manuscript. This project was supported financially by the hundreds top talents program awarded to Yan Li from the Chinese Academy of Sciences, the Major State Basic Research Development Program of China (No. 2009CB522300), the NSFC (No.81173076), and the project of recruited top talent of sciences and technology of Yunnan Province (2009C1120).

Issue Date: 11 February 2018

	Service

	E-mail this article
	E-mail Alert
	RSS
	Articles by authors

	Xing-Yao LI
	Yuan-Yuan WANG
	Chun-Mao YUAN
	Xiao-Jiang HAO
	Yan LI

Trendmd:

Cite this article:

Xing-Yao LI,Yuan-Yuan WANG,Chun-Mao YUAN, et al. A reporter gene system for screening inhibitors of Wnt signaling pathway[J]. Natural Products and Bioprospecting, 2013, 3(1): 24-28.

URL:

http://npb.kib.ac.cn/EN/10.1007/s13659-012-0094-0 OR http://npb.kib.ac.cn/EN/Y2013/V3/I1/24

[1]	Muller, J. M.; Chevrier, L.; Cochaud, S.; Meunier, A. C.; Chadeneau, C. Drug Discov. Today Dis. Mech. 2007, 4, 285-291.
[2]	Klaus, A.; Birchmeier, W. Nat. Rev. Cancer 2008, 8, 387-398.
[3]	Clevers, H.; Nusse, R. Cell 2012, 149, 1192-1205.
[4]	de Sousa, E. M.; Vermeulen, L.; Richel, D.; Medema, J. P. Clin. Cancer Res. 2011, 17, 647-653.
[5]	van Es, J. H.; Jay, P.; Gregorieff, A.; van Gijn, M. E.; Jonkheer, S.; Hatzis, P.; Thiele, A.; van den Born, M.; Begthel, H.; Brabletz, T.; Taketo, M. M.; Clevers, H. Nat. Cell Biol. 2005, 7, 381-386.
[6]	Brabletz, T.; Jung, A.; Dag, S.; Hlubek, F.; Kirchner, T. Am. J. Pathol. 1999, 155, 1033-1038.
[7]	Ceballos, M. P.; Parody, J. P.; Alvarez Mde, L.; Ingaramo, P. I.; Carnovale, C. E.; Carrillo, M. C. Biochem. Pharmacol. 2011, 82, 1682-1691.
[8]	Wang, Y.; Krivtsov, A. V.; Sinha, A. U.; North, T. E.; Goessling, W.; Feng, Z.; Zon, L. I.; Armstrong, S. A. Science 2010, 327, 1650-1653.
[9]	Lu, D.; Zhao, Y.; Tawatao, R.; Cottam, H. B.; Sen, M.; Leoni, L. M.; Kipps, T. J.; Corr, M.; Carson, D. A. Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 3118-3123.
[10]	Chien, A. J.; Moore, E. C.; Lonsdorf, A. S.; Kulikauskas, R. M.; Rothberg, B. G.; Berger, A. J.; Major, M. B.; Hwang, S. T.; Rimm, D. L.; Moon, R. T. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 1193-1198.
[11]	Wang, L.; Heidt, D. G.; Lee, C. J.; Yang, H.; Logsdon, C. D.; Zhang, L.; Fearon, E. R.; Ljungman, M.; Simeone, D. M. Cancer Cell 2009, 15, 207-219.
[12]	Vaira, S.; Friday, E.; Scott, K.; Conrad, S.; Turturro, F. J. Cell Physiol. 2012, 227, 578-586.
[13]	Ilyas, M.; Tomlinson, I. P.; Rowan, A.; Pignatelli, M.; Bodmer, W. F. Proc. Natl. Acad. Sci. U. S. A. 1997, 94, 10330-10334.
[14]	Kim, J.; Zhang, X.; Rieger-Christ, K. M.; Summerhayes, I. C.; Wazer, D. E.; Paulson, K. E.; Yee, A. S. J. Biol. Chem. 2006, 281, 10865-10875.
[15]	Shtutman, M.; Zhurinsky, J.; Simcha, I.; Albanese, C.; D'Amico, M.; Pestell, R.; Ben-Ze'ev, A. Proc. Natl. Acad. Sci. U. S. A. 1999, 96, 5522-5527.
[16]	Tetsu, O.; McCormick, F. Nature 1999, 398, 422-426.
[17]	Itokawa, H.; Qiao, Z. S.; Hirobe, C.; Takeya, K. Chem. Pharm. Bull. 1995, 43, 1171-1175.
[18]	Cragg, G. M.; Grothaus, P. G.; Newman, D. J. Chem. Rev. 2009, 109, 3012-3043.
[19]	Newman, D. J.; Cragg, G. M. J. Nat. Prod. 2012, 75, 311-335.
[20]	Thorne, N.; Inglese, J.; Auld, D. S. Chem. Biol. 2010, 17, 646-657.

[1]	Jin-Tang Cheng, Shi-Tao Chen, Cong Guo, Meng-Jiao Jiao, Wen-Jin Cui, Shu-Hui Wang, Zhe Deng, Chang Chen, Sha Chen, Jun Zhang, An Liu. *Triterpenoid Saponins from the Seeds of Aesculus chinensis* and Their Cytotoxicities**[J]. Natural Products and Bioprospecting, 2018, 8(1): 47-56.
[2]	Zhen-Nan Ye, Mu-Yuan Yu, Ling-Mei Kong, Wei-Hua Wang, Yuan-Feng Yang, Jie-Qing Liu, Ming-Hua Qiu, Yan Li. Biflavone Ginkgetin,a Novel Wnt Inhibitor,Suppresses the Growth of Medulloblastoma[J]. Natural Products and Bioprospecting, 2015, 5(2): 91-97.
[3]	Jing Zhang, Ling-Mei Kong, Rui Zhan, Zhen-Nan Ye, Jian-Xin Pu, Han-Dong Sun, Yan Li. *Two Natural ent-kauranoids as Novel Wnt Signaling Inhibitors*[J]. Natural Products and Bioprospecting, 2014, 4(3): 135-140.
[4]	Mounir Tilaoui, Hassan Ait Mouse, Abdeslam Jaafari, Abdelmajid Zyad. Differential Effect of Artemisinin Against Cancer Cell Lines[J]. Natural Products and Bioprospecting, 2014, 4(3): 189-196.
[5]	Xu DENG, Ling-Mei KONG, Yu ZHAO, Juan HE, Li-Yan PENG, Yan LI, Qin-Shi ZHAO. Exploring of drug leads from diversity-oriented Michael-acceptor library derived from natural products[J]. Natural Products and Bioprospecting, 2012, 2(5): 210-216.
[6]	Regina M. S. ARAÚJO, Antônio F. M. VAZ, Jaciana S. AGUIAR, Luana C. B. B. COELHO, Patrícia M. G. PAIVA, Ana M. M. MELO, Teresinha G. SILVA, Maria T. S. CORREIA. *Lectin from Crataeva tapia* bark exerts antitumor, antiinflammtory and analgesic activities**[J]. Natural Products and Bioprospecting, 2011, 1(2): 97-100.

Viewed

Full text

Abstract

Cited

Shared

Discussed