| ORIGINAL ARTICLES |
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Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata |
| Ni Huang1,2, Yi-Na Yang1,3, Jia Huang1, Hui-Yan Shao1, Yan-Lang Li1, Shi-Hui Qin1,3, Han-Fen Li1,2, Xiao-Jiang Shen1, Liu Yang1, Jiang-Miao Hu1 |
1. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing, 100049, China;
3. College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, Anhui, China |
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Abstract Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 103 and 1.25 × 104 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.
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| Keywords
Dictyophora rubrovalvata
Polysaccharides
Structure characterization
Immunoactivity
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| Fund:Key Project of Yunnan Provincial Nature Foundation (202401AS070075), the authors declare no competing interests. |
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Corresponding Authors:
Liu Yang,E-mail:yangliu@mail.kib.ac.cn;Jiang-Miao Hu,E-mail:hujiangmiao@mail.kib.ac.cn
E-mail: yangliu@mail.kib.ac.cn;hujiangmiao@mail.kib.ac.cn
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Issue Date: 13 December 2024
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