| ORIGINAL ARTICLES |
|
|
|
|
|
Polysaccharide fraction from Triplostegia glandulifera Wall and its renoprotective effect in streptozotocin-induced diabetic mice by attenuating oxidative stress |
| Hai-Hui Guo1, Lei Wu1, Dan Mi1, Xing-Yu Zhang1, Fu-Mei He1, Ting Lei1,2, Fu-Sheng Wang1,2 |
1. College of Pharmacy, Dali University, Xueren Road 2, Xiaguan, Dali, 671000, Yunnan Province, People's Republic of China;
2. Yunnan Key Laboratory of Screening and Research On Anti-Pathogenic Plant Resources From Western Yunnan, Dali, 671000, People's Republic of China |
|
|
|
|
Abstract Triplostegia glandulifera Wall (T. glandulifera) is an ethnomedicine commonly used by ethnic minorities in Yunnan, China, to treat kidney disease. However, there are few reports on the renoprotective effects of this substance, and the active ingredients remain unclear. In this study, we extracted the polysaccharide fractions TGB and TGC using the water extraction-alcohol precipitation method and determined their molecular weight (Mw) and monosaccharide composition. The study investigated the protective effects of TGB and TGC fractions against diabetic nephropathy (DN) using an in vitro high glucose-induced HRMCs model and an in vivo STZ-induced diabetic mouse model. HPLC analysis revealed that TGB contained D-galacturonic acid, D-glucose, D-galactose, and D-arabinose, and had a lower Mw than TGC. In vitro, TGB showed concentration-dependent antioxidant activity and effectively reduced abnormal proliferation and while attenuating oxidative stress in HRMCs. In mice with diabetes, TGB corrected the dysregulation of glucose-lipid metabolism and alleviated oxidative stress in the kidneys. Additionally, it improved renal function and reduced renal tissue damage. The study suggests that the low Mw polysaccharides (TGB) have better activity against DN through the antioxidative stress mechanism.
|
| Keywords
Triplostegia glandulifera
Diabetic nephropathy
Oxidative stress
Streptozotocin
Human renal mesangial cells
|
| Fund:National Natural Science Foundation of China, 31860098, Fu-Sheng Wang, 82260679, Fu-Sheng Wang |
|
Corresponding Authors:
Ting Lei,E-mail:leiting@dali.edu.cn;Fu-Sheng Wang,E-mail:wfsyn@163.com
E-mail: leiting@dali.edu.cn;wfsyn@163.com
|
|
Issue Date: 14 October 2024
|
|
|
[1] Stehouwer CDA. Microvascular dysfunction and hyperglycemia: a vicious cycle with widespread consequences. Diabetes. 2018;67:1729-41.
[2] Sifuentes-Franco S, Padilla-Tejeda DE, Carrillo-Ibarra S, Miranda-Díaz AG. Oxidative stress, apoptosis, and mitochondrial function in diabetic nephropathy. Int J Endocrinol. 2018;2018:1-13.
[3] Luan JJ. Protective Effects of Astragalosides on Kidney in Experimental Diabetic Animal Model and Its Potential Mechanism [Doctoral dissertation]. Anhui Medical University; 2012.
[4] Umanath K, Lewis JB. Update on diabetic nephropathy: core curriculum 2018. Am J Kidney Dis. 2018;71:884-95.
[5] Bikbov B, Purcell CA, Levey AS, Smith M, Abdoli A, Abebe M, et al. Global, regional, and national burden of chronic kidney disease, 1990-2017: a systematic analysis for the global burden of disease study 2017. The Lancet. 2020;395:709-33.
[6] Wen Y, Tu YY, Huang Q, Liu R, Liu J, Zhang FY, et al. Moringa oleifera Lam seed extract protects kidney function in rats with diabetic nephropathy by increasing GSK-3β activity and activating the Nrf2/HO-1 pathway. Phytomedicine. 2022. https://doi.org/10.1016/j.phymed.2021.153856.
[7] Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother. 2005;59:365-73.
[8] Qi SS, Shao ML, Ze S, Zheng HX. Salidroside from Rhodiola rosea L. attenuates diabetic nephropathy in STZ induced diabetic rats via anti-oxidative stress, anti-inflammation, and inhibiting TGF-β1/Smad pathway. J Funct Foods. 2021. https://doi.org/10.1016/j.jff.2020.104329.
[9] Yang BL, Yu HX. Yi nationality pharmacy of China. Kunming: Yunnan Nationalities Publishing House; 2004.
[10] Li GD, He TC. Yi Nationality Plant Medicine. Chengdu: Sichuan People Publishing House; 1990.
[11] Kunming Military Region Logistics Department Health Department. Yunnan ZhongcaoYao Xuan. Tianjin: Tianjin People’s Printing Factory; 1970.
[12] Dali Prefecture People’s Government. Resources annals of traditional Chinese medicine in Dali. Kunming: Yunnan Nationalities Publishing House; 1991.
[13] Liu XB, Guo MX, Shi GR, Fang CS, Liu GM. Mechanisms of Hypoglycemic Effects of Triplostegia grandifolra. Anhui Nongye Kexue. 2012;40:16111-2.
[14] Luan JJ, Li WP, Han J, Zhang W, Gong HL, Ma R. Renal protection of in vivo administration of tempol in streptozotocin-induced diabetic rats. J Pharmacol Sci. 2012;119:167-76.
[15] Liu XB, Guo MX, Li LX, Zhao Y, Wang FS. Research on the hypoglycemic effect of Triplostegia grandifolra. Yunnan J Tradit Chin Med Mater Medica. 2008;49:50.
[16] Wang PC, Zhao S, Yang BY, Wang QH, Kuang HX. Anti-diabetic polysaccharides from natural sources: a review. Carbohydr Polym. 2016;148:86-97.
[17] Sun CX, Chen Y, Li XZ, Tai GH, Fan YY, Zhou YF. Anti-hyperglycemic and anti-oxidative activities of ginseng polysaccharides in STZ-induced diabetic mice. Food Funct. 2014;5:845.
[18] Jianfang F, Yuan J, YanYang T, Jufang F, Zhang N, Gao B, et al. A polysaccharide from Acanthopanax senticosus improves the antioxidant status in alloxan-induced diabetic mice. Carbohydr Polym. 2012;88:517-21.
[19] Fu JF, Fu JF, Liu Y, Li RY, Gao B, Zhang NY, et al. Modulatory effects of one polysaccharide from Acanthopanax senticosus in alloxan-induced diabetic mice. Carbohydr Polym. 2012;87:2327-31.
[20] Sun LQ, Wang CH, Shi QJ, Ma CH. Preparation of different molecular weight polysaccharides from Porphyridium cruentum and their antioxidant activities. Int J Biol Macromol. 2009;45:42-7.
[21] Powell DW, Kenagy DN, Zheng S, Coventry SC, Xu J, xiang, Cai L, et al. Associations between structural and functional changes to the kidney in diabetic humans and mice. Life Sci. 2013;93:257-64.
[22] Gounden V, Bhatt H, Jialal I. Renal function tests statpearls treasure Island (FL). Petersburg: StatPearls Publishing; 2024.
[23] Sun HJ, Wu ZY, Cao L, Zhu MY, Liu TT, Guo L, et al. Hydrogen sulfide: recent progression and perspectives for the treatment of diabetic nephropathy. Molecules. 2019;24:2857.
[24] Hosseini A, Abdollahi M. Diabetic neuropathy and oxidative stress: therapeutic perspectives. Oxid Med Cell Longev. 2013;2013:1-15.
[25] Hasheminasabgorji E, Jha JC. Dyslipidemia, diabetes and atherosclerosis: role of inflammation and ROS-redox-sensitive factors. Biomedicines. 2021;9:1602.
[26] Gu W, Wang X, Zhao HF, Geng JL, Li XL, Zheng KJ, et al. Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway. Eur J Pharmacol. 2022;918: 174776.
[27] Sun LQ, Wang L, Zhou Y. Immunomodulation and antitumor activities of different-molecular-weight polysaccharides from Porphyridium cruentum. Carbohydr Polym. 2012;87:1206-10.
[28] Lo TC-T, Chang CA, Chiu K-H, Tsay P-K, Jen J-F. Correlation evaluation of antioxidant properties on the monosaccharide components and glycosyl linkages of polysaccharide with different measuring methods. Carbohydr Polym. 2011;86:320-7.
[29] Huang GL, Chen X, Huang HL. Chemical modifications and biological activities of polysaccharides. Curr Drug Targets. 2016;17:1799-803.
[30] Chiu C-H, Peng C-C, Ker Y-B, Chen CC, Lee A, Chang W-L, et al. Physicochemical characteristics and anti-inflammatory activities of antrodan, a novel glycoprotein isolated from Antrodia cinnamomea Mycelia. Molecules. 2013;19:22-40.
[31] Zhang WN, Su RN, Gong LL, Yang WW, Chen J, Yang R, et al. Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb. seeds. Carbohydr Polym. 2019;209:363-71.
[32] Kao T-H, Chen B-H. Functional components in soybean cake and their effects on antioxidant activity. J Agric Food Chem. 2006;54:7544-55.
[33] Li HF, Xu J, Liu YM, Ai SB, Qin F, Li ZW, et al. Antioxidant and moisture-retention activities of the polysaccharide from Nostoc commune. Carbohydr Polym. 2011;83:1821-7. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
