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LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
| Leonardo Mendes de Souza Mesquita1, Caroline Fabri Bittencourt Rodrigues1, Cláudia Quintino da Rocha1,2, Mayara Silveira Bianchim1, Clenilson Martins Rodrigues3, Vanda Maria de Oliveira3, Henrique Hessel Gaeta1, Mariana Novo Belchor1, Marcos Hikari Toyama1, Wagner Vilegas1 |
1 Laboratory of Bioprospection of Natural Products(LBPN), UNESP-Saõ Paulo State University/Coastal Campus of Saõ Vicente, Pça Infante Dom Henrique S/N, Saõ Vicente, Saõ Paulo CEP:11330-900, Brazil;
2 Laboratório de Estudos Avançados em Fitomedicamentos(LEAF), UFMA-Federal University of Maranhaõ, Av. dos Portugueses, 1966-Bacanga, Saõ Luís, Maranhaõ CEP:65080-805, Brazil;
3 Embrapa Agroenergy, Brazilian Agricultural Research Corporation, W3 Norte, PqEB, Brasília, DF 70770-901, Brazil |
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Abstract We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis[liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved[LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series:one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z[288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of-16 Da (homologous series of afzelechins-m/z 873-3465 Da) and + 16 Da (homologous series of gallocatechins-m/z 905-3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series:glucogallocatechins (m/z 779-3371) and glucoafzelechins (m/z 747-3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 lg mL-1) have a morphine-like effect and intense anti-inflammatory activity.
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| Keywords
Catechins
Tannins
Morphine-like effect
Anti-inflammatory
Mangroves
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| Fund:This work was supported by Fundaçaõ de Amparo à Pesquisa do Estado de Saõ Paulo (FAPESP) (Grant 2009/52237-9 to WV, fellowship 2014/23951-3 to LMSM). |
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Corresponding Authors:
Wagner Vilegas
E-mail: wagner.vilegas@unesp.br
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Issue Date: 28 January 2019
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| Cite this article: |
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Leonardo Mendes de Souza Mesquita,Caroline Fabri Bittencourt Rodrigues,Cláudia Quintino da Rocha, et al. LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties[J]. Natural Products and Bioprospecting,
2019, 9(1): 23-34.
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| URL: |
| http://npb.kib.ac.cn/EN/10.1007/s13659-018-0192-8 OR http://npb.kib.ac.cn/EN/Y2019/V9/I1/23 |
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