| ORIGINAL ARTICLES |
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Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway |
| Yuru Shi, Xiaoqian Zhang, Shengji Pei, Yuhua Wang |
| Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Heilongtan, Kunming, 650201, Yunnan, China |
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Abstract Adenosma buchneroides Bonati, also known as fleagrass, is an important medicinal plant used by the Akha (Hani) people of China for treating inflammation-related skin swelling, acne, and diarrhoea, among other conditions. In this study, we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne. The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro, and decreased the release of NO, IL-6, TNF-α, and IL-10 in the LPS-induced RAW264.7 macrophages. The levels of proteins associated with the nuclear factor-kappa B (NF-κB) pathway were examined by western blotting and immunofluorescence, demonstrating that fleagrass downregulated the expression of TLR4, MyD88, NF-κB/p65, and iNOS and blocked the nuclear translocation of NF-κB/p65. Furthermore, fleagrass exhibited acute anti-inflammatory activity in paw oedema models. The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation, suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.
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| Keywords
Fleagrass
Anti-inflammatory activities
TLR4/MyD88/NF-κB signaling pathway
Ethnopharmacology
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| Fund:This work was funded by Plant Germplasm Resources Innovation Project of Chinese Academy of Sciences (No. KFJ-BRP-007-002) and Yunnan Revitalization Talent Support Program Industrial Innovation Talent Project (Fleagrass Planting and Mosquito Repellent Product Industrialization Research and Development). |
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Corresponding Authors:
Yuhua Wang,E-mail:wangyuhua@mail.kib.ac.cn
E-mail: wangyuhua@mail.kib.ac.cn
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Issue Date: 14 October 2024
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