| ORIGINAL ARTICLE |
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Antiproliferative polyketides from fungus Xylaria cf. Longipes SWUF08-81 in different culture media |
| Kittiwan Sresuksai1, Sasiphimol Sawadsitang1, Phongphan Jantaharn1, Pakin Noppawan2, Audomsak Churat1, Nuttika Suwannasai3, Wiyada Mongkolthanaruk4, Thanaset Senawong5, Sarawut Tontapha6, Pairot Moontragoon6,7, Vittaya Amornkitbamrung6,7, Sirirath McCloskey1 |
1. Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry (PERCH-CIC), Khon Kaen University, Khon Kaen, 40002, Thailand;
2. Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand;
3. Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand;
4. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand;
5. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand;
6. Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand;
7. Institute of Nanomaterials Research and Innovation for Energy (IN-RIE), Khon Kaen University, Khon Kaen, 40002, Thailand |
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Abstract Bioactive compounds from the wood-decay fungus Xylaria cf. longipes SWUF08-81, cultivated in three different culture media (GM, YM and PDB), were isolated. Their structures and stereochemistry were deduced from spectroscopic and MS data analysis, together with quantum chemical calculations of 13C NMR chemical shifts and electronic circular dichroism (ECD) spectra. Five undescribed polyketides including dibenzofuran (1), mellein (2), dihydroisocoumarin (15), and two pyrans (16, 17), together with twenty-three compounds were determined. Compounds 18 and 20 were significantly toxic against cancer cell lines (HCT116, HT29, MCF-7 and HeLa) based on the MTT assay. Quantification by HPLC showed that 18 was produced three-fold higher in the broth of PDB than YM. These studies showed that the production of different compounds were primarily dependent on nutrition sources and it has given a starting point for the growth optimization conditions for the scaling up of bioactive compounds production.
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| Keywords
Xylaria
Dibenzofuran
Asperentin
Pyran
Antiproliferative activity
HPLC analysis
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| Fund:This research was supported by the Fundamental Fund of Khon Kaen University. We would like to gratefully acknowledge the Development and Promotion of Science and Technology Talents Project (DPST) and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation, for their financial support. |
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Corresponding Authors:
Sirirath McCloskey,E-mail:sirsod@kku.ac.th
E-mail: sirsod@kku.ac.th
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Issue Date: 19 February 2024
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