| 2023-6-25 |
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Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights |
| Luan Wen1,2, Zhou-Wei Wu1,2, Li-Wu Lin1,2, Abdulbaset Al-Romaima1,2, Xing-Rong Peng1,2, Ming-Hua Qiu1,2 |
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China;
2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China |
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Abstract Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw=10.1 kDa) and MCPc (Mw=5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
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| Keywords
Lepidium meyenii
Polysaccharide
Molecular weight
Structural characterization
α-Glucosidase inhibition
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| Fund:The research work was financially supported by the National Natural Science Foundation of China (No. 31872675), as well as the Cooperation Project with DR PLANT Company (2023). |
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Corresponding Authors:
Ming-Hua Qiu,E-mail:mhchiu@mail.kib.ac.cn
E-mail: mhchiu@mail.kib.ac.cn
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Issue Date: 18 July 2023
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