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Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity |
| Ganna Kravchenko1, Oksana Krasilnikova1, Ain Raal2, Matar Mazen1, Natalia Chaika1, Igor Kireyev1, Andriy Grytsyk3, Oleh Koshovyi1 |
1 National University of Pharmacy, 53 Pushkinska Str., Kharkiv 61002, Ukraine;
2 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia;
3 Ivano-Frankivsk National Medical University, 2 Halytska Str., Ivano-Frankivsk 76018, Ukraine |
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Abstract Bearberry (Arctostaphylos uva-ursi L.) is a perennial plant of the heather family (Ericaceae). The leaves are dominated by arbutin, phenol carbonic acids flavonoids, saponins, etc. It was previously shown that the bearberry leaves extract reduced blood glucose level in healthy animals under glucose overload, so it need to be studied more detail. The aim of the study was to investigate the chemical composition and the effect of dry alcohol extract from bearberry leaves, which enriched with cysteine, on the rats pancreas under experimental dexamethasone-induced insulin resistance (IR). Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation were obtained according to the developed method with 50% ethanol solution. Their phytochemical profile, hypoglycaemic and pancreatic protective effect were investigated. Phenologlycoside (arbutin), phenolic carboxylic acid (gallic acid), 5 flavonoids and 4 hydroxycinnamic acids were identified and quantified in the extracts by HPLC. Present data revealed that bearberry leaves alcoholic dry extract enriched with cysteine has a hypoglycaemic and pancreatic protective effect in treated animals under dexamethasone-induced IR model. Treatment improved hyperglycaemia, insulin resistance and beta cell reduction induced by dexamethasone injections.
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| Keywords
Arctostaphylos uva-ursi L.
Extract
Hypoglycaemic activity
Leaves
Phenolic substances
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| Fund:The research was funded by the Ministry of Health Care of Ukraine at the expense of the State Budget in the framework#2301020"Scientific and scientific-technical activity in the field of health protection "on the topic" Modern approaches to the creation of new medicines for a correction of metabolic syndrome". |
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Corresponding Authors:
Ain Raal,E-mail:ain.raal@ut.ee
E-mail: ain.raal@ut.ee
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Issue Date: 12 October 2022
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