| ORIGINAL ARTICLES |
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Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest |
| Rong-Fang Mu1,4, Yan-Fen Niu3, Qian Wang1,4, Hui-Min Zhou1,4, Jing Hu1, Wan-Ying Qin1,4, Wen-Yong Xiong1,2,4 |
1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China;
2 Key Laboratory of Medicinal Chemistry for Natural Resource, Yunnan University, Kunming 650500, Yunnan, People's Republic of China;
3 Biomedical Engineering Research Center, Kunming Medical University, Kunming 650500, Yunnan, People's Republic of China;
4 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China |
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Abstract Eriocalyxin B, an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx, has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory. However, the function and mechanism of the compound in adipocyte differentiation is still unknown. Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets, triglycerides and the expressions of adipogenesis-related factors, including C/EBPβ, C/EBPα, PPARγ, and FABP4. Moreover, we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation, most likely by suppressing mRNAs and proteins of CDK1, CDK2, Cyclin A and Cyclin B1. Overall, we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.
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| Keywords
Eriocalyxin B
Adipocyte differentiation
Cell cycle
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| Fund:Thanks Dr. Jing Hu, Yan-Ting Lu and Fang Wang for discussing the work. This work was supported by National Key R&D Program of China, (2017YFC1700906) and Yunnan Provincial Science and Technology Department, China (2017FA044 and 2013HA023). |
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Corresponding Authors:
Wen-Yong Xiong, Jing Hu
E-mail: xiong.wenyong@mail.kib.ac.cn,xwy@ynu.edu.cn;hujing@mail.kib.ac.cn
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Issue Date: 02 June 2020
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