A Novel Insulinotropic Peptide from the Skin Secretions of <i>Amolops loloensis</i> Frog

doi:10.1007/s13659-014-0037-z

Natural Products and Bioprospecting

2014, Vol. 4

Issue (5) : 309-313 DOI: 10.1007/s13659-014-0037-z

A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

Guo-Xiang Mo¹, Xue-Wei Bai¹, Zong-Jie Li¹, Xiu-Wen Yan¹, Xiao-Qing He², Ming-Qiang Rong²

1. School of Biological Sciences, Nanjing Agriculture University, Nanjing 210095, Jiangshu, China;
2. Kunming Institue of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China

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Abstract Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog, such as antimicrobial peptides, bradykinin-like peptides and algesic peptides. A novel insulinotropic peptide named amolopin was identified in A. loloensis frog's skin secretion. Its primary structure sequence was determined by Edman degradation as: FLPIVGKSLSGLSGKL-NH2. BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues, including mastoparan, exendins and a-latrotoxin, nor does it like incretins(e.g. glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide) either. However, amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica. Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner. Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca²⁺. In conclusion, a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A. loloensis frog. Further work is necessary to evaluate its potential as novel anti-diabetic candidate.

Keywords Insulinotropic peptide Amolops loloensis Skin secretion Insulin-releasing Frog

Fund:This work was supported by National Natural Science Foundation(31200590), Jiangsu Province(BK2012365, BE2012748) and Yunan Province(2013FB072).

Issue Date: 11 February 2018

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	Xiao-Qing He
	Ming-Qiang Rong

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Guo-Xiang Mo,Xue-Wei Bai,Zong-Jie Li, et al. A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog[J]. Natural Products and Bioprospecting, 2014, 4(5): 309-313.

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http://npb.kib.ac.cn/EN/10.1007/s13659-014-0037-z OR http://npb.kib.ac.cn/EN/Y2014/V4/I5/309

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