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Comparative Study on “Long-Dan”, “Qin-Jiao” and Their Adulterants by HPLC Analysis |
| Fang-Fang Liu1,2, Yan-Ming Wang1,4, Hong-Tao Zhu1, Dong Wang1, Chong-Ren Yang1, Min Xu1, Ying-Jun Zhang1 |
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China;
2. Yunnan University of Traditional Chinese Medicine, Kunming 650500, People's Republic of China;
3. University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China |
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Abstract "Long-Dan" and "Qin-Jiao" are two important TCM herbs since ancient times in China. In the Chinese Pharmacopoeia, the dried roots and rhizomes of four species from the genus Gentiana, e.g. Gentiana manshurica, G. scabra, G. triflora and G. rigescens, are recorded under the name of Gentianae Radix et Rhizoma("Long-Dan" in Chinese), while the other four species from the same genus including G. macrophylla, G. crassicaulis, G. straminea and G. duhurica are recorded and used as the raw materials of Gentianae Macrophyllae Radix("Qin-Jiao" in Chinese). On the basis of the establishment of a validated HPLC-UV method for quantifying simultaneously, five iridoid glycosides, e.g. loganic acid(1), swertiamarinin(2), gentiopicroside(3), sweroside(4) and 2'-(o, m-dihydroxybenzyl) sweroside(5) have been used successfully as chemical markers for the comparison of the species used as "Long-Dan", "Qin-Jiao" and their adulterants in the present study. The results suggested that four iridoid glycosides 1-4 commonly existed in both "Long-Dan" and "Qin-Jiao", while 2'-(o, m-dihydroxybenzyl) sweroside(5) also existed as one of the major components in "Dian-Long-Dan" species. Moreover, the contents of compounds 1-5 were various in different "LongDan" and "Qin-Jiao" species. Herein, we profiled and compared three "Long-Dan" species, four "Qin-Jiao" species and five adulterants by applying multivariate statistical techniques to their HPLC data sets to establish the differences and/or similarities.
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| Keywords
"Long-Dan"
"Qin-Jiao"
Gentiana
HPLC analysis
Iridoid glycosides
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| Fund:This work was supported by Science and Technology Planning Project of Yunnan Province(2010CD106), the 973 Program of Ministry of Science and Technology of P. R. China(2011CB915503), the State Key Laboratory of Phytochemistry and Plant Resources in West China, KIB, CAS(P2010-ZZ03) and The Fourteenth Candidates of the Young Academic Leaders of Yunnan Province(Min XU, 2011CI044). |
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Issue Date: 11 February 2018
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