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Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways |
| Donatus Wewura Adongo1, Charles Kwaku Benneh1, Augustine Tandoh1, Robert Peter Biney2, Kennedy Kwami Edem Kukuia3, Priscilla Kolibea Mante4, Benjamin Kingsley Harley5, David Oteng1, Emmanuel Aduboffour Appiah1, Ernest Cudjoe Anorbor1, Eric Woode1 |
1. Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana;
2. Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana;
3. Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu, Accra, Ghana;
4. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;
5. Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana |
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Abstract Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL-1), fluoxetine (3×10-5 mg mL-1) and diazepam (1.5×10-7 mg mL-1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract’s anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1×10-3 mg mL-1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.
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| Keywords
Anxiety disorders
Pseudospondias microcarpa
Zebrafish
Novel tank
Benzodiazepines
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| Fund:We appreciate the technical support provided by Messrs. R. Agbolosoo and B. Cato of the Department of Pharmacology and Toxicology at the University of Health and Allied Sciences (UHAS). |
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Corresponding Authors:
Donatus Wewura Adongo,E-mail:dadongo@uhas.edu.gh
E-mail: dadongo@uhas.edu.gh
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Issue Date: 03 November 2023
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