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The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans |
| Jie Yang1,2,3, Qin-Li Wan1,2, Quan-Zhang Mu1, Chun-Feng Wu1, Ai-Jun Ding1,2, Zhong-Lin Yang1,2, Ming-Hua Qiu1, Huai-Rong Luo1 |
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China |
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Abstract Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine(TCM), some were claimed to slow down aging. Qingyangshen(Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B(Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor(daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.
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| Keywords
Otophylloside B
Caenorhabditis elegans
Aging
DAF-16/FOXO
IIS signaling pathway
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| Fund:This work was partially supported by the Yunnan provincial government(20080A007), and the 100 Talents Program of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany(P2008-ZZ21 and T2009-KF05). |
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Issue Date: 11 February 2018
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