Regular Article |
|
|
|
|
|
Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant |
Ranabir SAHU, Saikat DEWANJEE |
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India |
|
|
Abstract Solenostemon scutellarioides is a commercially important ornamental plant. In present study, it was attempted to establish a protocol for high frequency in vitro regeneration of S. scutellarioides. Caulogenesis was found to be significant in solid MS medium supplemented with 0.5 mg dm-3 BAP. Combination of GA3(0.5 mg dm-3) and BAP(0.5 mg dm-3) induced high frequency regeneration coupled with higher plant height. The plantlets exposed to IBA(1 mg dm-3) exhibited significant root development in terms of first appearance of root and number of roots per shoot. To improve its commercial acceptability in terms of plant architecture and foliage colouration, two weeks old in vitro grown plantlets were exposed to different culture conditions namely MS strength, sucrose concentration, pH and light. A variable plant types with diverse ornamental traits were developed under different culture conditions.
|
Keywords
anthocyanin
chlorophyll
in vitro
micropropagation
Solenostemon scutellarioides
|
Fund:The authors are thankful to University Grants Commission, New Delhi, India for providing fund to Mr Ranabir Sahu through Rajiv Gandhi National Fellowship, 2011. |
Issue Date: 11 February 2018
|
|
|
[1] |
Garcia, J. F.; O'Neil, R. J. Biol. Control 2000, 18, 225-234.
|
[2] |
Cantazaro, C.; Bhatti, S.; Muhammad, S.; Abdullah, S. Hortscience 2005, 40, 993-1147.
|
[3] |
Oren-Shamir, M.; Nissim-Levi, A.; Ovadia, R.; Kagan, S.; Shaked, S. Acta Horticult. 2003, 624, 171-176.
|
[4] |
Nguyen, P.; Cin, V. D. Plant Physiol. Bioch. 2009, 47, 934-945.
|
[5] |
Lebowitz, R. Plant Breeding Review 1985, 3, 343-360.
|
[6] |
Rife, D. C. J. Hered. 1948, 39, 85-91.
|
[7] |
Yoshida, K.; Kawachi, M.; Mori, M.; Maeshima, M.; Kondo, M.; Nishimura, M.; Kondo, T. Plant Cell Physiol. 2005, 46, 407-415.
|
[8] |
Mol, J.; Grotewold, E.; Koes, R. Trends Plant Sci. 1998, 3, 212-217.
|
[9] |
Murashige, T.; Skoog, F. Physiol. Plant. 1962, 15, 473-497.
|
[10] |
Coello, C. Y.; Miceli, C. L.; Orantes, C.; Dendooven, L.; Gutiérrez, F. A. Gayana Bot. 2010, 67, 19-26.
|
[11] |
Dube, P.; Gangopadhyay, M.; Dewanjee, S.; Ali, M. N. Indian J. Biotechnol. 2011, 10, 228-231.
|
[12] |
Bhattacharya, R.; Ray, A.; Gangopadhyay, M.; Bhattacharya, S. Plant Cell Biotechnol. Mol. Biol. 2008, 8, 39-46.
|
[13] |
DeMason, D. A. Planta 2005, 222, 151-166.
|
[14] |
Bialecka, B.; Kepcznski. J. Plant Growth Regul. 2007, 51, 21-31.
|
[15] |
Sahoo, Y.; Pattnaik, S. K.; Chand, P. K. In Vitro Cell. Dev. Biol. Plant 1997, 33, 293-296.
|
[16] |
Gaspar, T. H.; Kevers, C.; Penel, C.; Greppin, H.; Reid D. M.; Thorpe, T. A. In Vitro Cell. Dev. Biol. Plant 1996, 32, 272-289.
|
[17] |
Beena, M. R.; Martin, K. P.; Kirti, P. B.; Hariharan, M. Plant Cell Tiss. Org. 2003, 72, 285-289.
|
[18] |
Sreekumar S.; Seeni S.; Pushpangadan P. Plant Cell Tiss. Org. 2000, 62, 211-218.
|
[19] |
Close, D. C.; Beadle, C. I. The Bot. Rev. 2003, 69, 149-161.
|
[20] |
Singh, A.; Selvi, M.; Sharma, R. J. Exp. Bot. 1999, 50, 1619-1625.
|
[21] |
Murray, J. R.; Hackett, W. P. Plant Physiol. 1991, 97, 343-351.
|
[22] |
Goncalves, J.; Antonio, R.; Vieira, M.; Vieira, G. Rev. Bras. Fisiol. Veg. 2001, 13, 149-157.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|