| ORIGINAL ARTICLES |
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UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay |
| Jian-Gang Zhang1, Xiao-Yan Huang1, Yun-Bao Ma1, Ji-Jun Chen1,2, Chang-An Geng1 |
1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory of Natural Medicinal Chemistry, 132#Lanhei Road, Kunming 650201, Yunnan, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China |
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Abstract Uncariae Ramulus Cum Uncis (Gou-Teng), the dried hook-bearing stems of several Uncaria plants (Rubiaceae), is a wellknown herbal medicine in China. The clinical application of Gou-Teng is bewildered for the morphological and chemical similarity between different species. In order to discern their chemical and biological difference, an ultra-fast liquid chromatography equipped with ion trap time-of-flight mass spectrometry (UFLC-IT/TOF-MS) combining with melatonin (MT1 and MT2) and 5-hydroxytryptamine (5-HT1A and 5-HT2C) receptors agonistic assay in vitro was conducted on seven Uncaria species. As a result, 57 compounds including 35 indole alkaloids, ten flavonoids, five triterpenoids, five chlorogenic analogues, and two other compounds were characterized based on their MS/MS patterns and UV absorptions. Specifically, cadambine-type and corynanthein-type alkaloids were exclusively present in U. rhynchophylla and U. scandens, whereas corynoxine-type alkaloids were commonly detected in all the seven Uncaria plants. Three Uncaria species, U. rhynchophylla, U. macrophylla, and U. yunnanensis showed obviously agnostic activity on four neurotransmitter receptors (MT1, MT2, 5-HT1A, and 5-HT2C). This first-time UFLCMS-IT-TOF analyses integrated with biological assay on seven Uncaria plants will provide scientific viewpoints for the clinical application of Gou-Teng.
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| Keywords
Uncariae Ramulus Cum Uncis
Uncaria plants
LCMS-IT-TOF analyses
Melatonin and 5-hydroxytryptamine receptors
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| Fund:This work was financially supported by the National Natural Science Foundation of China (81573322), the Yunnan Wanren Project (YNWR-QNBJ-2018-061), the Youth Innovation Promotion Association, CAS (2013252), the Program of Yunling Scholarship, the Yunnan Science Fund for Excellent Young Scholars (2019FI017), and the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province. |
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Corresponding Authors:
Chang-An Geng
E-mail: gengchangan@mail.kib.ac.cn
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Issue Date: 29 February 2020
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