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Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways |
| Chuanchuan Cai1, Si Liu2,3, Yufeng Liu2,3, Shaobin Huang2,3, Shiya Lu4, Fang Liu2,3, Xiaohua Luo2,3, Christos C. Zouboulis5, Ge Shi2,3 |
1. Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China;
2. Department of Cosmetic and Plastic Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
3. Biomedical Innovation Center, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
4. Huamei-Bond International College, Guangzhou, 510520, China;
5. Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, 06847, Dessau, Germany |
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Abstract Insulin-like growth factor-1 (IGF-1) is considered as a pathogenic factor contributing to sebaceous gland dysfunction, which leads to acne vulgaris. Paeoniflorin (Pae), a bioactive monomer derived from total glycosides of paeony, has shown potential in treating various diseases. However, its anti-acne effects on human sebocytes are not well understood. In this study, we investigated the effects of Pae on acne development induced by IGF-1 in SZ95 sebocytes. Following IGF-1 stimulation, SZ95 sebocytes were exposed to Pae and then determined for proliferation, cell cycle, apoptosis, lipogenesis and pro-inflammatory cytokine secretion. We also analyzed the expression of proteins involved in the PI3K/Akt/FoxO1 and JAK2/STAT3 pathways. In vitro experiments demonstrated that Pae significantly inhibited colony formation, induced G1/S cell cycle arrest, promoted apoptosis, inhibited lipogenesis and cytokine synthesis in IGF-1-treated SZ95 sebocytes. Furthermore, Pae suppressed the phosphorylation of Akt, FoxO1, JAK2, and STAT3. Importantly, the sebo-suppressive and anti-inflammatory effects of Pae were enhanced by blocking PI3K and JAK2. In summary, our findings suggest that Pae has potent anti-proliferative and pro-apoptotic effects in SZ95 sebocytes. Additionally, Pae effectively protects against IGF-1-induced lipogenesis and inflammation by targeting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways.
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| Keywords
Paeoniflorin
Acne vulgaris
Sebocytes
Insulin-like growth factor-1
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| Fund:This work was s financially supported by the National Natural Science Foundation of China (Project No. 81773345 and 82104856) and Basic and Applied Basic Research Foundation of Guangdong Province (No. SL2023A04J01298). |
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Corresponding Authors:
Ge Shi,E-mail:shig9@mail.sysu.edu.cn
E-mail: shig9@mail.sysu.edu.cn
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Issue Date: 13 December 2024
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