| ORIGINAL ARTICLES |
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Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A |
| Ya-Ya Yang1, Shun-Li Jing1, Jia-Li Shao1, Ji-Xuan Chen1, Wei-Feng Zhang1, Si-Yuan Wan2, Yu-Ping Shen1, Huan Yang1, Wei Yu2 |
1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu Province, People's Republic of China;
2. Development Department, Jiangsu Grand Xianle Pharmaceutical Co., Ltd, Yancheng, 224555 People's Republic of China |
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Abstract Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl-γ-aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β-glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products.
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| Keywords
Immobilized β-glucosidase
Modified SBA-15
Sagittatoside A
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| Fund:This work was supported by the National Natural Science Foundation of China (Grant No: 81873196). |
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Corresponding Authors:
Huan Yang,E-mail:yanghuan1980@ujs.edu.cn;Wei Yu,E-mail:13784052172@163.com
E-mail: yanghuan1980@ujs.edu.cn;13784052172@163.com
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Issue Date: 13 December 2024
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