| ORIGINAL ARTICLES |
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Bioassay-guided isolation of cytotoxic constituents from the fowers of Aquilaria sinensis |
| Jun Yang1, Dong-Bao Hu2, Meng-Yuan Xia1, Ji-Feng Luo1, Xing-Yu Li3, Yue-Hu Wang1 |
1 Key Laboratory of Economic Plants and Biotechnology and Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People’s Republic of China;
2 School of Chemical Biology and Environment, Yuxi Normal University, Yuxi 653100, People’s Republic of China;
3 College of Science, Yunnan Agricultural University, Kunming 650201, People’s Republic of China. |
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Abstract Bioassay-guided fractionation of the EtOH extract from the flowers of Aquilaria sinensis (Lour.) Spreng. (Thymelaeaceae) led to the isolation of a new cucurbitane-type triterpenoid, aquilarolide A (1), along with five known compounds (2–6). The structure of 1 was elucidated by extensive 1D and 2D nuclear magnetic resonance (NMR) experiments and mass spectrometry (MS) data and theoretical calculations of its electronic circular dichroism (ECD) spectra. Aquilarolide A, cucurbitacin E (3), cucurbitacin B (4), and 7-hydroxy-6-methoxy-2-[2-(4-methoxyphenyl)ethyl]-4H-1-benzopyran-4-one (6) showed significant cytotoxicity against human lung adenocarcinoma SPC-A-1, human lung squamous cell carcinoma NCI-H520, human lung adenocarcinoma A549, and paclitaxel-resistant A549 (A549/ Taxol) cell lines. All four active compounds, with IC50 values ranging from 0.002 to 0.91 μM, had better inhibitory activities against A549/Taxol cells than paclitaxel (IC50 = 1.80 μM). Among them, cucurbitacin E (IC50 = 0.002 μM) is the most active. Further studies are needed to evaluate their in vivo antitumor activities and to clarify their mechanisms.
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| Keywords
Thymelaeaceae
Aquilaria sinensis
Paclitaxel-resistant lung cancer cells
Cucurbitane-type triterpenoids
2-(2-Phenylethyl)chromones
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| Fund:This study was supported by Beijing Sino-Science Aquilaria Technology Co., Ltd., Beijing, China (Grant No. KET202101). |
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Corresponding Authors:
Yue-Hu Wang, E-mail:wangyuehu@mail.kib.ac.cn
E-mail: wangyuehu@mail.kib.ac.cn
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Issue Date: 26 April 2022
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