| ORIGINAL ARTICLE |
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Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives |
| Dong-Qiong Yang1,2, Zhao-Rong Chen3, Duo-Zhi Chen1, Xiao-Jiang Hao1, Shun-Lin Li1 |
1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China;
3 Tianjin Agricultural University, Tianjin 300380, People's Republic of China |
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Abstract Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional halfleaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by westernblot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.
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| Keywords
Amaryllidaceae alkaloids
Lycoricidine derivatives
Lycoris radiata
Tobacco mosaic virus Anti-TMV activity
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| Fund:This work was financially supported by grants from the National Natural Science. Foundation of China (Nos. 31770389, 31470427, 31270404). |
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Corresponding Authors:
Shun-Lin Li
E-mail: lisl@mail.kib.ac.cn
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Issue Date: 02 June 2018
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