Pyrocincholic acid 3<i>β-O-β</i>-D-quinovopyranosyl-28-<i>O-β</i>-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes

doi:10.1007/s13659-017-0127-9

Natural Products and Bioprospecting

2017, Vol. 7

Issue (3) : 225-234 DOI: 10.1007/s13659-017-0127-9

Pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes

Li Peng^1,2, Yanting Lu^1,2, Yuhui Xu^1,2, Jing Hu^1,2, Fang Wang^1,2, Yumei Zhang³, Wenyong Xiong^1,4

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
2. University of the Chinese Academy of Sciences, Beijing 100049, China;
3. Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
4. Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, China

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Abstract Obesity is crucially involved in many metabolic diseases, such as type 2 diabetes, cardiovascular disease and cancer. Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity. In this study, we investigated the effect of pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside (PAQG), a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma, on adipogenesis and lipid metabolism in 3T3-L1 adipocytes. The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6 days in differentiation process. PAQG significantly reduced the adipogenesis, adiponectin secretion and the expression level of key transcription factors related to adipogenesis, such as PPARγ, C/EBPβ, C/EBPα, and FABP4. Moreover, PAQG increased the levels of FFA and glycerol in medium and reduced TG level in mature adipocytes. Interestingly, PAQG not only promoted the activation of AMPK and genes involved in fatty oxidation including PDK4 and CPT1a, but also inhibited those genes involved in fatty acid biosynthesis, such as SREBP1c, FAS, ACCa and SCD1. In conclusion, PAQG inhibits the differentiation and regulates lipid metabolism of 3T3-L1 cells via AMPK pathway, suggesting that PAQG may be a novel and promising natural product for the treatment of obesity and hyperlipidemia.

Keywords Pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside Adipogenesis Lipid metabolism AMP-activated protein kinase

Issue Date: 06 February 2018

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	Yanting Lu
	Yuhui Xu
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	Fang Wang
	Yumei Zhang
	Wenyong Xiong

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Li Peng,Yanting Lu,Yuhui Xu, et al. Pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes[J]. Natural Products and Bioprospecting, 2017, 7(3): 225-234.

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http://npb.kib.ac.cn/EN/10.1007/s13659-017-0127-9 OR http://npb.kib.ac.cn/EN/Y2017/V7/I3/225

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