| ORIGINAL ARTICLES |
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Xiaoyankangjun tablet alleviates dextran sulfate sodium-induced colitis in mice by regulating gut microbiota and JAK2/STAT3 pathway |
| Suqin Yang1, Jingtao Huang2, Wenjing Tan1, Xiankun Xia1, Dali Gan1, Yalei Ren1, Hanwen Su2, Meixian Xiang1 |
1. School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, Hubei, People's Republic of China;
2. Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China |
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Abstract Xiaoyankangjun tablet (XYKJP) is a traditional Chinese medicine formulation used to treat intestinal disorders in clinical practice. However, the specific therapeutic mechanism of action of XYKJP in colitis has not yet been elucidated. This study aimed to reveal the multifaceted mechanisms of action of XYKJP in treating colitis. The model established based on DSS-induced colitis in C57BL/6 mice was employed to estimate the effect of XYKJP on colitis, which was then followed by histological assessment, 16S rRNA sequencing, RT-qPCR, ELISA, and Western blot. XYKJP alleviated the symptoms of DSS-induced colitis mainly by reducing oxidative stress, inflammatory responses, and intestinal mucosal repair in colitis tissues. In addition, XYKJP regulated the intestinal flora by increasing the relative abundance of Akkermansia and Bifidobacterium and reducing the relative abundance of Coriobacteriaceae_UCG-002. Mechanistically, XYKJP increased the content of short-chain fatty acids (SCFAs) in the feces, particularly propanoic acid and butyric acid, activated their specific receptor GPR43/41, furthermore activated the Nrf2/HO-1 pathway, and suppressed the JAK2/STAT3 pathway. XYKJP significantly alleviated the symptoms of experimental colitis and functioned synergistically by regulating the intestinal flora, increasing the production of SCFAs, and activating their specific receptors, thereby repressing oxidative stress and inflammation.
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| Keywords
Xiaoyankangjun tablet
Colitis
Nrf2/HO-1 pathways and JAK2/STAT3 pathways
Tight junction proteins
Gut microbiota
GPR43/41 receptor
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| Fund:This work was supported by the National Natural Science Foundation of China (81902141), the Knowledge Innovation Program of Wuhan-Basic Research (2022020801010402), The Fundamental Research Funds for the Central Universities South-Central MinZu University (Grant Numbers: CZZ24017 and CSZY22002), and the Fundamental Research Funds for Health Commission of Hubei Province (ZY2023M022); The Natural Science Foundation of Hubei Province (2024AFD252, 2022CFB127). |
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Corresponding Authors:
Hanwen Su,E-mail:Suhanwen-1@163.com;Meixian Xiang,E-mail:xiangmeixian@mail.scuec.edu.cn
E-mail: Suhanwen-1@163.com;xiangmeixian@mail.scuec.edu.cn
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Issue Date: 14 October 2024
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