| ORIGINAL ARTICLES |
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Petrosamine isolated from marine sponge Petrosia sp. demonstrates protection against neurotoxicity in vitro and in vivo |
| Joana Ribeiro1, Henrique Araújo-Silva1, Mário Fernandes1, Joilna Alves da Silva2, Francisco das Chagas L. Pinto3, Otília Deusdenia L. Pessoa3, Hélcio Silva Santos2,3, Jane Eire Silva Alencar de Menezes2, Andreia C. Gomes1 |
1. CBMA (Centre of Molecular and Environmental Biology)/Aquatic Research Network (ARNET) Associate Laboratory, Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal;
2. Program in Natural Sciences, Natural Products Chemistry Laboratory, State University of Ceará, Fortaleza, Ceará, Brazil;
3. Department of Organic and Inorganic Chemistry, Science Center, Federal University of Ceará, Fortaleza, Ceará, Brazil |
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Abstract According to The World Alzheimer Report 2023 by Alzheimer’s Disease International (ADI) estimates that 33 to 38.5 million people worldwide suffer from Alzheimer’s Disease (AD). A crucial hallmark associated with this disease is associated with the deficiency of the brain neurotransmitter acetylcholine, due to an affected acetylcholinesterase (AChE) activity. Marine organisms synthesize several classes of compounds, some of which exhibit significant AChE inhibition, such as petrosamine, a coloured pyridoacridine alkaloid. The aim of this work was to characterize the activity of petrosamine isolated for the first time from a Brazilian marine sponge, using two neurotoxicity models with aluminium chloride, as exposure to aluminium is associated with the development of neurodegenerative diseases. The in vitro model was based in a neuroblastoma cell line and the in vivo model exploited the potential of zebrafish (Danio rerio) embryos in mimicking hallmarks of AD. To our knowledge, this is the first report on petrosamine’s activity over these parameters, either in vitro or in vivo, in order to characterize its full potential for tackling neurotoxicity.
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| Keywords
Petrosamine
Petrosia sp.
Neuroprotection
Aluminium-induced neurotoxicity
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| Fund:This work was supported by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P. https://doi.org/10.54499/UIDB/04050/2020. MF (SFRH/BD/147819/2019) holds a scholarship from FCT. FUNCAP-INTERNACIONALIZAÇÃO (Grant ITR-0214-00060.01.00/23), CNPq-Universal (Grant 406119/2021-0). |
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Corresponding Authors:
Jane Eire Silva Alencar de Menezes,E-mail:jane.menezes@uece.br；Andreia C. Gomes,E-mail:agomes@bio.uminho.pt
E-mail: jane.menezes@uece.br;agomes@bio.uminho.pt
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Issue Date: 16 May 2024
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