Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

doi:10.1007/s13659-012-0017-0

Natural Products and Bioprospecting

2012, Vol. 2

Issue (2) : 87-91 DOI: 10.1007/s13659-012-0017-0

Regular Article

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

Ramona LIEDER^a,b, Sigrídur Thóra REYNISDÓTTIR^a, Finnbogi THORMÓDSSON^e, Chuen-How NG^c, Jon Magnús EINARSSON^c, Jóhannes GÍSLASON^c, Jóhannes BJÖRNSSON^d,f, Sveinn GUDMUNDSSON^a, Pétur Henry PETERSEN^e, Ólafur Eysteinn SIGURJÓNSSON^a,b,d

a The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland;
b School of Science and Engineering, Reykjavik University, Menntavegi 1, 101 Reykjavik, Iceland;
c Genis ehf, Vatnagördum 18, 104 Reykjavik, Iceland;
d Biomedical Center, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
e Department of Anatomy, Medical Faculty, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
f Department of Pathology, Landspitali University Hospital, Baronsstig, 101 Reykjavik, Iceland

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Abstract Human mesenchymal stem cells(hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins(CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

Keywords YKL-40 mesenchymal stem cells osteogenic differentiation chitinase-like-protein

Fund:This work was supported by grants from Technology Development Fund, managed by the Icelandic Center for Research, and Landspitali University Hospital Research Fund.

Issue Date: 11 February 2018

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	Ramona LIEDER
	Sigrídur Thóra REYNISDÓTTIR
	Finnbogi THORMÓDSSON
	Chuen-How NG
	Jon Magnús EINARSSON
	Jóhannes GÍSLASON
	Jóhannes BJÖRNSSON
	Sveinn GUDMUNDSSON
	Pétur Henry PETERSEN
	Ólafur Eysteinn SIGURJÓNSSON

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Ramona LIEDER,Sigrídur Thóra REYNISDÓTTIR,Finnbogi THORMÓDSSON, et al. Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation[J]. Natural Products and Bioprospecting, 2012, 2(2): 87-91.

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http://npb.kib.ac.cn/EN/10.1007/s13659-012-0017-0 OR http://npb.kib.ac.cn/EN/Y2012/V2/I2/87

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